【佳学基因检测】使用靶向序列捕获增强病毒组测序

肿瘤基因检测费17800说明

开会学习医学博士年度肿瘤汇报《肿瘤基因易感位点列表及发生率分析》《Sci Adv》在 2022 Mar; 8(9): eabm5386发表了一篇题目为《使用靶向序列捕获增强病毒组测序》肿瘤靶向药物治疗基因检测临床研究文章。该研究由Igor Stevanovski, Data curation, Formal analysis, Investigation, Methodology, Resources, Software, Validation,  , Sanjog R. Chintalaphani, Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Software, Validation, Visualization, Writing - original draft, Writing - review & editing,  Hasindu Gamaarachchi, Formal analysis, Investigation, Methodology, Software, Validation, Writing - original draft,  James M. Ferguson, Formal analysis, Resources, Software,  Sandy S. Pineda, Conceptualization, Investigation, Methodology, Supervision, Visualization, Writing - review & editing,  ,  Carolin K. Scriba, Investigation, Resources,   Michel Tchan, Resources, Writing - review & editing,  Victor Fung, Investigation, Resources, Writing - review & editing,  Karl Ng, Resources, Validation, Writing - review & editing,  Andrea Cortese, Conceptualization, Investigation, Resources, Validation, Writing - review & editing,  ,  Henry Houlden, Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Software, Supervision, Validation, Visualization, Writing - original draft, Writing - review & editing,  ,  Carol Dobson-Stone, Investigation, Validation, Writing - review & editing,  Lauren Fitzpatrick, Investigation, Resources,  Glenda Halliday, Funding acquisition, Investigation, Resources, Supervision, Validation, Writing - review & editing,  Gianina Ravenscroft, Funding acquisition, Resources, Writing - review & editing,  Mark R. Davis, Resources,  Nigel G. Laing, Conceptualization, Funding acquisition, Investigation, Project administration, Resources, Supervision, Writing - review & editing,  ,  Avi Fellner, Writing - review & editing,  Marina Kennerson, Conceptualization, Resources, Validation, Writing - review & editing,   Kishore R. Kumar, Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Visualization, Writing - review & editing,   and Ira W. Deveson, Conceptualization, Data curation, Formal analysis, Funding acquisition, Methodology, Project administration, Supervision, Validation, Visualization, Writing - original draft, Writing - review & editing等完成。促进了对人体内及环境中未知病毒的快速和准确检测，有利于更清晰地鉴定出疾病的发病原因。

病毒种属鉴别及精准治疗临床研究内容关键词：

宏基因组鸟枪法测序,MSS,靶向序列捕获面板,ViroCap,一种计算方法

病毒种属鉴别靶向治疗基因检测临床应用结果

宏基因组鸟枪法测序 (MSS) 是表征病毒种群的重要工具。它独立于培养，不需要样本中病毒的先验知识，并且可以提供有用的基因组信息。然而，MSS 可能缺乏敏感性，并且可能产生的数据不足以进行详细分析。我们创建了一个靶向序列捕获面板 ViroCap，旨在从感染脊椎动物宿主的 34 个家族的 DNA 和 RNA 病毒中富集核酸。一种计算方法将约 10 亿 bp 的病毒参考序列压缩成 <2 亿 bp 的独特的、具有代表性的序列，适用于靶向序列捕获。我们比较了在标准 MSS 中检测病毒与靶向序列捕获后的 MSS 中检测病毒的有效性。首先，我们分析了两组样本，一组来自提交给诊断病毒学实验室的样本，另一组来自儿童发烧研究中收集的样本。我们在两组中检测到 14 和 18 种病毒，包括来自 10 个科的 19 个属，在捕获富集后显着增强了基因组表示。捕获后病毒读数百分比的中位数增加倍数分别为 674 和 296。第一组捕获后的中位覆盖广度从 2.1% 增加到 83.2%，第二组从 2.0% 增加到 75.6%。接下来，我们分析了包含一组不同的指环病毒序列的样本，并证明 ViroCap 可用于检测与用于选择捕获探针的参考相比变异高达 58% 的病毒序列。 ViroCap 显着增强了一套全面病毒的 MSS，并具有研究和临床应用的实用性。

肿瘤发生与复发转移国际数据库描述：

Metagenomic shotgun sequencing,MSS, targeted sequence capture panel, ViroCap, A computational approach