【佳学基因检测】人参皂甙抑制舌癌细胞迁移和侵袭所需要的基因检测结果
1年靶向药物要多少钱评价
探索明白《J Appl Oral Sci》在. 2022 Sep 2;30:e20220144.发表了一篇题目为《人参皂甙Rd通过H19/miR-675-5p/CDH1轴抑制舌癌细胞迁移和侵袭》肿瘤靶向药物治疗基因检测临床研究文章。该研究由Lu Chang, Dongxu Wang, Shaoning Kan, Ming Hao, Huimin Liu, Zhijing Yang, Qianyun Xia, Weiwei Liu 等完成。促进了肿瘤的精准治疗与个性化用药的发展,进一步强调了基因信息检测与分析的重要性。
肿瘤靶向药物及精准治疗临床研究内容关键词:
人参皂甙,miR-675-5p,CDH1,抑制,舌癌细胞,迁移,侵袭,基因检测结果
肿瘤靶向治疗基因检测临床应用结果
舌鳞状细胞癌转移抑制基因检测研究目的:舌鳞状细胞癌(TSCC)是一种口腔癌,恶性程度高,早期迁移和侵袭频繁。只有少数药物可以治疗舌癌。 Ginsen总生存期ide Rd 是一种具有抗癌作用的人参提取物。许多非编码RNA在舌癌中异常表达,从而影响其发生和发展。 H19和miR-675-5p可以促进癌细胞生长。本研究旨在分析人参皂甙Rd对舌癌H19和miR-675-5p的调控作用。舌鳞状细胞癌转移抑制基因检测研究方法:采用CCK8和流式细胞术研究其生长和凋亡。 Transwell测定用于评估侵袭;评估迁移的伤口愈合试验;和集落形成测定以测试细胞形成集落的能力。通过 qPCR 分析 H19、miR-675-5p 和 CDH1 表达。使用蛋白质印迹检测 E-钙粘蛋白表达。采用CRISPR/cas9系统敲除CDH1。舌鳞状细胞癌转移抑制基因检测研究结果:人参皂甙Rd抑制SCC9细胞生长,增加细胞凋亡。 Ginsen总生存期ide Rd 还抑制 SCC9 细胞的迁移和侵袭。 H19 和 miR-675-5p 高表达,而 CDH1 和 E-cadherin 表达低。 H19 和 miR-675-5p 促进 SCC9 转移。相反,CDH1 和 E-cadherin 抑制 SCC9 细胞的转移。生物信息学分析显示miR-675-5p与CDH1相关。人参皂苷Rd处理后H19和miR-675-5p表达降低,而CDH1和E-cadherin表达增加。舌鳞状细胞癌转移抑制基因检测研究结论:人参皂苷Rd通过H19/miR-675-5p/CDH1轴抑制舌癌细胞迁移和侵袭。
肿瘤发生与复发转移国际数据库描述:
Objective: Tongue squamous cell carcinoma (TSCC) is an oral cancer, with high malignancy and frequent early migration and invasion. Only a few drugs can treat tongue cancer. Ginsenoside Rd is a ginseng extract with anti-cancer effects. Many noncoding RNAs are abnormally expressed in tongue cancer, thus influencing its occurrence and development. H19 and miR-675-5p can promote cancer cell growth. This study aimed to analyze the regulation effect of ginsenoside Rd on H19 and miR-675-5p in tongue cancer.Methodology: We used CCK8 and flow cytometry to study the growth and apoptosis. Transwell assay was used to assess invasion; wound-healing assay to assess migration; and colony formation assays to test the ability of cells to form colonies. H19, miR-675-5p, and CDH1 expressions were analyzed by qPCR. E-cadherin expression was detected using western blot. CRISPR/cas9 system was used for CDH1 knockout.Results: Ginsenoside Rd inhibited the growth and increased the apoptosis of SCC9 cells. Ginsenoside Rd also inhibited the migration and invasion of SCC9 cells. H19 and miR-675-5p were highly expressed, while CDH1 and E-cadherin expressions were low. H19 and miR-675-5p promoted SCC9 metastasis. In contrast, CDH1 and E-cadherin inhibited the metastasis of SCC9 cells. Bioinformatics analysis showed that miR-675-5p was associated with CDH1. H19 and miR-675-5p expressions decreased after ginsenoside Rd treatment, while CDH1 and E-cadherin expressions increased.Conclusions: Ginsenoside Rd inhibits tongue cancer cell migration and invasion via the H19/miR-675-5p/CDH1 axis.
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