【佳学基因检测】金合欢素抑制人肝细胞癌细胞系的细胞增殖并诱导细胞凋亡

肿瘤基因检测是什么意思—答案

综述在《肿瘤致病基因检测与转移潜能分析》收录《Molecules》在. 2022 Aug 23;27(17):5361.发表了一篇题目为《金合欢素抑制人肝细胞癌细胞系的细胞增殖并诱导细胞凋亡》肿瘤靶向药物治疗基因检测临床研究文章。该研究由Manal Alfwuaires, Hany Elsawy, Azza Sedky等完成。促进了肿瘤的精准治疗与个性化用药的发展，进一步强调了基因信息检测与分析的重要性。

肿瘤靶向药物及精准治疗临床研究内容关键词：

状态3,金合欢素,细胞凋亡,肝细胞癌,增殖

肿瘤靶向治疗基因检测临床应用结果

人类肝细胞癌 (HCC) 是全球第五大常见癌症和第三大死亡原因。最近的证据表明，STAT3 调节 HCC 中的增殖、存活、转移和血管生成基因。抑制 STAT3 激活的新型药物可用于预防或治疗 HCC。我们使用功能性蛋白质组学肿瘤通路技术平台和多个 HCC 细胞系来研究金合欢素 (ACN) 对 STAT3 活化、蛋白激酶、磷酸酶、STAT3 调节基因产物和细胞凋亡的影响。发现 ACN 在 HCC 细胞中以剂量和时间依赖性方式抑制 STAT3 活化。上游激酶 c-Src、Janus 激活激酶 1 和 Janus 激活激酶 2 也被抑制。钒酸盐处理消除了 STAT3 的 ACN 抑制，表明酪氨酸磷酸酶活性的参与。发现 ACN 通过 STAT3 抑制抑制参与增殖、存活和血管生成的基因的蛋白质表达。 ACN 似乎是一种新型 STAT3 抑制剂，可能是用于治疗 HCC 和其他癌症的有前途的治疗化合物。金合欢素;细胞凋亡；肝细胞癌;增殖。

肿瘤发生与复发转移国际数据库描述：

Human hepatocellular carcinoma (HCC) is the fifth most common cancer and the third leading cause of death across the world. Recent evidence suggests that STAT3 regulates proliferative, survival, metastasis, and angiogenesis genes in HCC. Novel agents that suppress STAT3 activation can be used to prevent or treat HCC. We used a functional proteomics tumor pathway technology platform and multiple HCC cell lines to investigate the effects of acacetin (ACN) on STAT3 activation, protein kinases, phosphatases, products of STAT3-regulated genes, and apoptosis. ACN was found to inhibit STAT3 activation in a dose- and time-dependent manner in HCC cells. Upstream kinases c-Src, Janus-activated kinase 1, and Janus-activated kinase 2 were also inhibited. The ACN inhibition of STAT3 was abolished by vanadate treatment, suggesting the involvement of tyrosine phosphatase activity. ACN was found to suppress the protein expression of genes involved in proliferation, survival, and angiogenesis via STAT3 inhibition. ACN appears to be a novel STAT3 inhibitor and may be a promising therapeutic compound for application in the treatment of HCC and other cancers.Keywords: STAT3; acacetin; apoptosis; hepatocellular carcinoma; proliferation.