【佳学基因检测】自由流动区电泳促进 H1299 肺癌细胞异质亚群的蛋白质组学分析

基因检测费收费标准秘决

课题调研如何选择有效的靶向药物治疗《肿瘤靶向药物选择的基因突变标准》《Anal Chim Acta》在. 2022 Sep 22;1227:340306.发表了一篇题目为《自由流动区电泳促进 H1299 肺癌细胞异质亚群的蛋白质组学分析》肿瘤靶向药物治疗基因检测临床研究文章。该研究由Amir Sohail, Xiaoteng Jiang, Amir Wahid, Huiyu Wang, Chengxi Cao, Hua Xiao 等完成。促进了肿瘤的精准治疗与个性化用药的发展，进一步强调了基因信息检测与分析的重要性。

肿瘤靶向药物及精准治疗临床研究内容关键词：

细胞亚群,馏分制备,自由流动区电泳,肺癌,蛋白质组学

肿瘤靶向治疗基因检测临床应用结果

癌细胞通常是异质的，其亚群包含与其独特表型相关的不同生物学信息。然而，将异质细胞制备成亚群是非常具有挑战性的，尤其是基于它们的表面电荷状态。在这里，采用自由流动区电泳 (FFZE) 分离和分级分离肺细胞。对 FFZE 的实验参数，包括电压、运行速度和缓冲液 pH 值进行了优化，然后实施优化的方法来分离 MRC-5 和 H1299 细胞。正常肺细胞MRC-5可分为一个主峰和几个小峰。对于肺癌细胞系 H1299，分离出两个主要峰和几个次要峰。对那些准备好的主要峰进行异质亚群的定量蛋白质组学分析。对于 H1299 细胞，在第一个主峰中鉴定出 2076 种蛋白质，在第二个主峰中发现 1398 种蛋白质，与 FFZE 分离前相比，平均增加了 13%（n = 3）。此外，我们发现第一个主峰中的 H1299 细胞比第二个主峰中的细胞具有更高的迁移率。许多细胞迁移和转移相关蛋白，如 RHBDF2、TRIM59 和 ADAM17，仅在 FFZE 后 H1299 的第一个主峰中表达，表明我们的方法可以区分具有较高转移表型的癌细胞亚群。结果表明，FFZE可以有效分离异质细胞亚群，可用于综合分析不同癌症的独特表型。馏分制备；自由流动区电泳；肺癌;蛋白质组学。

肿瘤发生与复发转移国际数据库描述：

Cancer cells are usually heterogeneous, whose subpopulations contain distinct biological information that related to their unique phenotype. However, preparative separating heterogeneous cells into subpopulations is very challenging, especially based on their surface charge status. Here, free-flow zone electrophoresis (FFZE) was employed to separate and fractionate lung cells. The experimental parameters for FFZE, including voltage, running speed, and buffer pH, were optimized and the optimized method was then implemented to separate MRC-5 and H1299 cells. Normal lung cells MRC-5 could be separated into one major peak and few minor peaks. For lung cancer cell line H1299, two major peaks and a few minor peaks were fractionated. Those prepared major peaks were subjected to quantitative proteomics analysis for heterogeneous subpopulation. For H1299 cells, 2076 proteins were identified in the first major peak, and 1398 proteins were revealed in the second major peak, which increased by 13% on average (n = 3) when compared to that of before FFZE fractionation. Furthermore, we found that H1299 cells in the first major peak have higher mobility than the cells in the second one. Many cell mobility and metastasis related proteins, such as RHBDF2, TRIM59, and ADAM17, were solely expressed in the first major peak of H1299 after FFZE, indicating our method can distinguish cancer cell subpopulations with higher metastatic phenotype. Our results showed that FFZE can effectively fractionate heterogeneous cell subpopulations, which might be used for the comprehensive analysis of unique phenotypes of different cancers.Keywords: Cell subpopulations; Fraction preparation; Free-flow zone electrophoresis; Lung cancer; Proteomics.