【佳学基因检测】ALDH+ 肺腺癌干细胞中新型多组学表达图谱的鉴定和基于图谱的竞争性内源性 RNA 网络的元分析

病原微生物基因检测多少钱要点

体会癌的基因检测基因解码如何创新治疗认识到《Biomed Res Int》在. 2022 Aug 31;2022:9545609.发表了一篇题目为《ALDH+ 肺腺癌干细胞中新型多组学表达图谱的鉴定和基于图谱的竞争性内源性 RNA 网络的元分析》肿瘤靶向药物治疗基因检测临床研究文章。该研究由Wei Yang, Yong Liang, Yuanyuan Zheng, Haitao Luo, Xiaofei Yang, Furong Li等完成。促进了肿瘤的精准治疗与个性化用药的发展，进一步强调了基因信息检测与分析的重要性。

肿瘤靶向药物及精准治疗临床研究内容关键词：

肿瘤靶向治疗基因检测临床应用结果

ALDH+ H1975 肺腺癌干细胞 (LSC) 是在肺腺癌 (LUAD) 中发现的罕见细胞群。 LSCs 可以自我更新，驱动肿瘤的发生、生长、转移和复发，并且由于其对药物和化学疗法的内在抗性，也是预后不良的主要原因。因此，LSCs 是 LUAD 治疗的一个有希望的靶点。非编码 RNA (ncRNA)，包括 microRNA (miRNA)、长链非编码 RNA (lncRNA) 和环状 RNA (circRNA)，在人类癌症的发病机制中发挥着许多重要的调节功能，显示了全面了解其机制的必要性肺癌的发生。尽管如此，对许多已知转录本和信使 RNA (mRNA) 的研究已经产生了新的信息。 ncRNA 中的未知生物标志物以及与未知 ncRNA 和 mRNA 的系统和全面的相互关系可能会为 LUAD 的生物学提供进一步的见解。在此，我们鉴定了一组新的 ncRNA，包括 miRNA、lncRNA 和 circRNA，并使用严格的生物信息学流程获得了 LSC 和 ALDH-H1975 LUAD 肿瘤细胞 (LTC) 中 ncRNA 和 mRNA 的差异表达模式。通过对已识别景观的荟萃分析，构建了新的竞争性内源性 RNA (ceRNA) 网络，以揭示调节 LSC 和 LTC 标志的潜在分子机制。本研究总结了新型 ncRNA 以及差异表达的 ncRNA 在 LSC 和 LTC 活性中的基本作用。此外，该研究还为未来识别 LUAD 中的诊断、治疗和预后生物标志物提供了综合资源。

肿瘤发生与复发转移国际数据库描述：

ALDH+ H1975 lung adenocarcinoma stem cells (LSCs) are a rare cell population identified in lung adenocarcinoma (LUAD). LSCs can self-renew, drive tumor initiation, growth, metastasis, and recurrence and are also the predominant cause of poor prognosis due to their intrinsic resistance to drugs and chemotherapy. Consequently, LSCs are a promising target for LUAD therapy. Noncoding RNAs (ncRNAs), including microRNAs (miRNAs), long noncoding RNAs (lncRNAs), and circular RNAs (circRNAs), exert many significant regulatory functions in the pathogenesis of human cancers, showing the necessity for a comprehensive understanding of the mechanisms that underlie lung carcinogenesis. Nonetheless, research on many known transcripts and messenger RNAs (mRNAs) has already generated new information. Unknown biomarkers in ncRNAs and systematic and comprehensive interrelation with unknown ncRNAs and mRNAs may provide further insights into the biology of LUAD. Herein, a set of novel ncRNAs that include miRNAs, lncRNAs, and circRNAs were identified, and differentially expressed patterns of ncRNAs and mRNAs in LSCs and ALDH-H1975 LUAD tumor cells (LTCs) were obtained using stringent bioinformatics pipelines. Through a meta-analysis of the identified landscapes, novel competitive endogenous RNA (ceRNA) networks were constructed to reveal the potential molecular mechanisms that regulate the hallmarks of LSCs and LTCs. This study presents a summary of novel ncRNAs and the fundamental roles of differentially expressed ncRNAs implicated in the activity of LSCs and LTCs. In addition, the study also provides a comprehensive resource for the future identification of diagnostic, therapeutic, and prognostic biomarkers in LUAD.